Principle
This technique combines the technique of IEF, which separates proteins in a mixture according to charge isoelectric point, with the size separation technique of SDS-PAGE. When combined to give 2D PAGE, the most sophisticated analytical method for separating proteins available is obtained.
Gel preparation of Isoelectric focusing gel electrophoresis
The carrier ampholytes, riboflavin and acrylamide solution with covering suitable pH range mixture is poured over a glass plate (25×10cm) that contains a spacer. The 2nd glass plate is then placed on top of the first to form gel cassette. Gel is polymerized by photo polymerization by placing the […]
Principle
Isoelectric focusing gel electrophoresis is a separation of amphoteric substance such as protein, amino acids and peptides based on their different isoelectric point on supporting medium using electricity.
Introduction
Separation of protein is carried out using cross-linked polyacrylamide gel electrophoresis. This is due to molecular sieving property of polyacrylamide gel electrophoresis that slows the migration of protein according to their molecular weight. Proteins were originally separated on polyacrylamide gels that were polymerized in a glass tubes, approximately 7 mm in diameter and about 10cm […]
Cord blood is a human Blood present in the blood vessels at the time of delivery .The blood vessels contain pair of umbilical arteries and one umbilical vein. The blood between the foetus and placenta is carried by these blood vessels. The umbilical cord blood is rich in haematopoietic stem cells. The cord blood is […]
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